How To Use The CD117 Antibody

by | Jan 20, 2017 | Medical Facilities

The CD117 or C-kit is designed to recognize proteins with a molecular weight of 145 kDa. It is rabbit polyclonal antibody that doesn’t interfere with the binding of the c-kit to the SCF. However, it will precipitate the unoccupied and occupied form of the c-kit. When binding the stem cell factor (SCF) with the c-kit-encoded receptor (tyrosine kinase Type III), you can stimulate a wide variety of biochemical responses that will help you develop cellular proliferation, survival, or migration. The c-kit plays a necessary role in melanogenesis, hematopoiesis, and gametogenesis.

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The CD117 antibody is designed to be used for research and has no known clones. The immunogen is a synthetic peptide created from the cytoplasmic domain of CD117 or c-kit proteins. The isotype is the Rabbit IgG, and there is no determined epitope. It has a molecular weight of 145kDa and has been tested on humans.

It is designed for Immunohistochemistry applications. The procedure includes using Formalin-fixed or paraffin-embedded tissues. Slides must be deparaffinized with xylene or an appropriate alternative. When using concentrated versions, you should dilute it using a ratio of 1:100, though this is an estimate and will depend on usage. To retrieve the antigen, you’ll boil tissue sections using 10mM citrate buffers with a pH of 6.0. You should boil it for 10 to 20 minutes and allow it to cool for 20 minutes until it reaches room temperature. Likewise, incubation periods are 10 minutes while at room temperature, after it has boiled and cooled.

The positive control includes the tonsil, skin, and gastrointestinal stromal tumor with a cellular localization in the cytoplasm.

You can find it in pre-diluted forms, as well as three concentrated forms.

The CD117 is designed to help bind the SCF with the c-kit. Visit Spring Bioscience for more information and purchase options.

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